Molecular Biology

Detectors in Real Time PCR

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Real time PCR employs detector for the monitoring of the progress of reaction (amplification of DNA). Detectors used can be various fluorescence markers. Among them the most common and important ones includes Taqman Probe and SYBR Green. Taqman assay is a widely used RT-PCR assay because of its sensitivity and specificity. It is based on 5′–3′ exonuclease activity of Taq DNA polymerase. Nonspecific fluorescent dye SYBR works on principle that when SYBR dyes bind to double-stranded DNA, their fluorescence increases.