Instrumentation

Cell Disruption Methods(Enzymatic)

Cell Disruption is a process of releasing biological content from a cell or a process of obtaining intracellular fluid via methods that opens up cell wall. An overall goal in cell disruption is to obtain intracellular fluid without disrupting any of its components.

Use of digestive enzymes to achieve cell lysis is enzymatic method of cell disruption. Cell lysis or disruption can be carried out by digestive enzymes which will decompose the microbial cell wall.

Cell Disruption Methods (Mechanical)-2

Mechanical method of cell disruption can be divided into solid shear methods and liquid shear methods. The major principle of mechanical disruption method is cells are being subjected to high stress via pressure, abrasion with rapid agitation with beads, or ultrasound. Intensive cooling of suspension after treatment is required in order to remove heat generated by dissipation of mechanical energy.

Cell Disruption Methods (Mechanical)-1

Mechanical method of cell disruption can be divided into solid shear methods and liquid shear methods. The major principle of mechanical disruption method is cells are being subjected to high stress via pressure, abrasion with rapid agitation with beads, or ultrasound. Intensive cooling of suspension after treatment is required in order to remove heat generated by dissipation of mechanical energy.