Instrumentation

Extraction Buffer

Posted on
Extraction Buffer

Cell disruption process is carried out in presence of buffer which maintains the integrity of biomolecules within the cell so it is called Extraction Buffer. They are also called as recovery buffer as these are used during recovering the desired nucleic material, proteins etc. by disrupting the cells.

Instrumentation

Cell Disruption Methods(Enzymatic)

Posted on
Cell Disruption Methods (Enzymatic Methods)

Cell Disruption is a process of releasing biological content from a cell or a process of obtaining intracellular fluid via methods that opens up cell wall. An overall goal in cell disruption is to obtain intracellular fluid without disrupting any of its components.

Use of digestive enzymes to achieve cell lysis is enzymatic method of cell disruption. Cell lysis or disruption can be carried out by digestive enzymes which will decompose the microbial cell wall.

Instrumentation

Cell Disruption Methods (Chemical)

Posted on
Cell Disruption Methods (Chemical Methods)

Cell Disruption is a process of releasing biological content from a cell or a process of obtaining intracellular fluid via methods that opens up cell wall. An overall goal in cell disruption is to obtain intracellular fluid without disrupting any of its components.

Chemical methods involves use of detergents, solvents and enzymes.

Instrumentation

Cell Disruption Methods (Physical)

Posted on
Cell Disruption Methods (Physical Methods)

Cell Disruption is a process of releasing biological content from a cell or a process of obtaining intracellular fluid via methods that opens up cell wall. An overall goal in cell disruption is to obtain intracellular fluid without disrupting any of its components.

Physical methods includes use of osmotic shock, decompression, thermolysis etc.

Instrumentation

Cell Disruption Methods (Mechanical)-2

Posted on
Disruption of Cell (Mechanical Methods)

Mechanical method of cell disruption can be divided into solid shear methods and liquid shear methods. The major principle of mechanical disruption method is cells are being subjected to high stress via pressure, abrasion with rapid agitation with beads, or ultrasound. Intensive cooling of suspension after treatment is required in order to remove heat generated by dissipation of mechanical energy.

Instrumentation

Cell Disruption Methods (Mechanical)-1

Posted on
Disruption of Cell (Mechanical Methods)

Mechanical method of cell disruption can be divided into solid shear methods and liquid shear methods. The major principle of mechanical disruption method is cells are being subjected to high stress via pressure, abrasion with rapid agitation with beads, or ultrasound. Intensive cooling of suspension after treatment is required in order to remove heat generated by dissipation of mechanical energy.

Instrumentation

Cell Disruption

Posted on
Cell Disruption

Cell Disruption is a process of releasing the biological content from a cell. Cell disruption is process of obtaining intracellular fluid via methods that opens up cell wall.
Cell disruption is an essential initial preparative step for the purification of intracellular protein products. The disruption of cells is necessary for the extraction and retrieval of the desired products.

Instrumentation

Rotors in Centrifuge

Posted on
Rotors in centrifuge

A centrifuge rotor is rotating unit of the centrifuge, which has fixed holes drilled at an angle. Test tubes are placed inside these holes and rotor spins to aid in the separation of materials.

Rotor is the part of centrifuge for holding the tubes with solution for separation through centrifugation with help of centrifugal field.