The antigen- antibody interaction is bimolecular irreversible association between antigen and antibody. The association between antigen and antibody includes various non-covalent interactions between epitope (antigenic determinant) and variable region (VH/VL) domain of antibody in a similar manner to that in which proteins bind to their cellular receptors, or enzymes bind to their substrates.
Macrophages are specialized cells involved in detection, phagocytosis and destruction of bacteria. Macrophages, widely distributed in organs and connective tissue plays central roles in innate and adaptive immunity. In addition to ingesting microbes, macrophages also ingest dead host cells, including cells that die in tissues because of trauma or interrupted blood supply and neutrophils that accumulate at sites of infection.
Secondary immunofluorescence is a double-layer method in which two antibodies are used. Multiple secondary antibodies can bind a single primary antibody. This provides signal amplification by increasing number of fluorophore molecules per antigen.
Direct immunofluorescence test is used to detect unknown antigen in a cell or tissue by employing a known labeled antibody that interacts directly with unknown antigen. If antigen is present, it reacts with labeled antibody and the antibody coated antigen is observed under UV light of fluorescence microscope
Immunofluorescence is a technique that utilizes fluorescent-labeled antibodies to detect specific target antigens. It is based on use of specific antibodies which have been chemically conjugated to fluorescent dyes which binds directly or indirectly to cellular antigens.
T lymphocytes can be divided into : effector, memory, and regulatory cells. Each type performs a distinct function during an immune response to foreign antigens. Effectors cells include helper T cells and Cytotoxic T cells.
These are differentiated by the expression of unique cell surface markers, such as CD4 for helper T cells and CD8 for cytotoxic T cells.
T lymphocytes (T cells) are type of White blood cells which derive their letter designation from their site of maturation i.e. Thymus. T lymphocytes are divided into three cell types T helper (TH) cells, T cytotoxic (TC) cells and T regulatory (TREG) cells.
Neutralization tests are performed in vitro at cellular level including cells in the form of organs and at sub-cellular level. They assess efficacy of antibody fragments, or antibodies, to inhibit critical stages of activities which are generally deleterious to humans. These tests mostly employed to viruses and toxins.
Toxin neutralization tests are based on the principle that biological action of toxin is neutralized on reacting with specific neutralizing antibodies called antitoxins.
Virus neutralization Tests are serological tests to detect presence and magnitude of functional systemic antibodies (Monoclonal and polyclonal) that prevent infectivity of a virus. E.g. viral hemagglutination inhibition test. Neutralization of a virus is defined as loss of infectivity through reaction of virus with specific antibody i.e. neutralization of viruses by their specific antibodies are called virus neutralization tests.
Virus Neutralization Tests can be reversible neutralization or stable neutralization.
Neutralization tests are performed in vitro at cellular level including cells in the form of organs and at sub-cellular level. When a neutralizing antibody defends a cell from an antigen or infectious body by neutralizing any biological effect, it is termed “neutralization”.
In diagnostic immunology and virology laboratories, evaluation of neutralizing antibodies, which destroy the infectivity of viruses, can be measured by neutralization method.