Genetics and Gene Biology

Southern Blotting Process

Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules.
Steps in southern blotting process include:

DNA extraction.
Restriction Digestion or DNA Fragmentation.
Agarose Gel Electrophoresis
Denaturation and Depurination
Blotting
Baking and blocking
Hybridization
Washing
Detection

Southern Blotting

Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA fragments by gel electrophoresis and identified by labeled probe hybridization.
It combines transfer of electrophoresis separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization, which is a key process.

Gene Cloning (rDNA)

Gene cloning is the process of producing a large number of copies of gene or a given DNA sequence. The technique is also known as molecular cloning.
Breakage of DNA molecule at two desired places to isolate a specific DNA segment and then insert it in another DNA molecule at a desired position will give a product called recombinant DNA. And the technique is known as recombinant DNA technology/ genetic engineering/gene cloning.