Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules.
Steps in southern blotting process include:
Restriction Digestion or DNA Fragmentation.
Agarose Gel Electrophoresis
Denaturation and Depurination
Baking and blocking
Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA fragments by gel electrophoresis and identified by labeled probe hybridization.
It combines transfer of electrophoresis separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization, which is a key process.
Gene cloning is the process of producing a large number of copies of gene or a given DNA sequence. The technique is also known as molecular cloning.
Breakage of DNA molecule at two desired places to isolate a specific DNA segment and then insert it in another DNA molecule at a desired position will give a product called recombinant DNA. And the technique is known as recombinant DNA technology/ genetic engineering/gene cloning.
Gene cloning is process of locating and copying a gene of interest out of DNA. molecular technique in which a gene of interest is fused or incorporated into a self-replicating genetic element such as plasmid.